The C1 domain diacylglycerol binding site and the three pharmacophore filters defined for PKC. A) The required position of a hybrid donor/acceptor group (purple-dotted sphere). B) The space available for a hydrogen bond-accepting atom forming a key interaction with Gly 253 (red-dotted sphere). C) Hydrophobic groups (yellow-dotted spheres) in position to interact with the lipid bilayer. This figure was generated using PyMOL .