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Figure 4 | Journal of Cheminformatics

Figure 4

From: Visualizing chemical structure-subcellular localization relationships using fluorescent small molecules as probes of cellular transport

Figure 4

Visual inspection revealed three distinct cell-associated staining patterns exhibited by the styryl probes. A) Zoom in image of a single cell showing typical staining pattern of organelles. Note the nucleus in the middle is dark, and the surrounding cytoplasm is bright with local hot spots indicative of organelle staining. B) Hoechst (top left), FITC (top right), TRITC (bottom left) and Cy5 (bottom right) images corresponding to organelle staining probe. C) Chemical structure, logP and predicted cellular distribution data of the probe visualized in A and B. D) Zoom in image of a single cell showing typical staining pattern associated with cellular membranes. Note the probe distribution in the cytoplasm appears diffuse, with the brightest signal generally present at the cell periphery. E) Hoechst (top left), FITC (top right), TRITC (bottom left) and Cy5 (bottom right) images corresponding to membrane staining probe. F) Chemical structure, logP and predicted cellular distribution data of the probe visualized in D and E. G) Zoom in image of a single cell showing typical staining pattern of nuclear staining probe. Note the probe’s optical signal is brightest in the cell nucleus, with nucleolar labeling. H) Hoechst (top left), FITC (top right), TRITC (bottom left) and Cy5 (bottom right) images corresponding to nuclear staining probe. I) Chemical structure, logP and predicted cellular distribution data of the probe visualized in G and H.

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