Kinome tree constructed with a distance matrix based on fingerprint enrichment profiles after exclusion of kinases with 16 or fewer shared activities. Kinases were colored based on the classification of kinase groups as defined by Manning et al. The new tree shows good agreement with the tree constructed earlier. In particular, CDK and CLK kinases are grouped together (with only CDK6 being out of the cluster). Protein C kinases are slightly more spread over 2 small clusters, but as a whole still remain close in the new tree as well. Tyrosine kinases remain clustered together, in particular the Ephrin kinases.