Table 1 Instrumental operational conditions and measurement by LA–ICP–MS
From: LA-iMageS: a software for elemental distribution bioimaging using LA–ICP–MS data
Instrument settings | |
Nebulizer | Meinhard |
Spray chamber | Cyclonic |
RF power (W) | 1300 |
Nebulizer gas flow (L min−1) | 1.0 |
Auxiliary gas flow (L min−1) | 2.0 |
Data acquisition parameters | |
Reading mode | Peak hopping |
Detector mode | Pulse |
Sweeps | 3 |
Dwell time (ms) | 30 |
Integration time (ms) | 270 (for each point) |
Detector dead time (ns) | 60 |
Lens voltage (V) | Automatic mode |
Monitored isotopes | 12C, 63Cu and 31P |
Laser conditions | |
Wavelength of Nd:YAG laser (nm) | 213 |
Laser ablation intensity (%) | 50 |
Frequency (Hz) | 20 |
Spot size (µm) | 12 |
Scan speed (µm s−1) | 10 |
Resolution—X axis (µm) | 2.7 |
Resolution—Y axis (µm) | 15 |