Fig. 6
CGS21680 and compounds 1–6 elevated cAMP in A2AR stably expressed in CHO-K1 cells, which were antagonized by ZM241385. A2AR stably expressed in CHO-K1 cells (CHO-K1-A2AR) were stimulated for 30 minutes with: CGS21680 (a), compound 1 (b), compound 2 (c), compound 3 (d), compound 4 (e), compound 5 (f), or compound 6 (g), after which the cAMP levels were determined. Subsequently compounds were antagonized with either 100 pM, 10 nM or 1 µM ZM241385, which decreased the cAMP levels to the same level of CHO-K1 cells (no A2AR stably expressed). Data represented are relative to the response of CGS21680, ± SEM of 4–9 individual replicates